| 产品包括 | 体积 | ||||
|---|---|---|---|---|---|
| 10X dsDNase Buffer | 370 µl | ||||
| dsDNase | 37 µl |
产品信息
The SignalStar Fluorescence Removal Kit is intended for use following the SignalStar™ Multiplex IHC Assay Manual protocol or the SignalStar™ Multiplex IHC Assay for Use on BOND RX Fully Automated Research Stainer by Leica Biosystems protocol. Continue to follow all guidance and recommendations from the original protocol when using the SignalStar Fluorescence Removal Kit.
For the SignalStar™ Multiplex IHC Assay Manual protocol:
1. After the second round of image acquisition, soak slides in dH2O for >30 min to gently remove coverslip.
2. Combine reagents to create the SignalStar Fluorescence Removal Solution:
a. For 5 slides: 75 µL 10X dsDNase Buffer, 7.5 µL dsDNase, and 667.5 µL Nuclease-free Water #12931.
b. For 10 slides: 150 µL 10X dsDNase Buffer, 15 µL dsDNase, and 1,335 µL Nuclease-free Water #12931.
3. 用封口膜覆盖并涡旋混合 10 秒。配制溶液时，所有 SignalStar 试剂盒组分均储存于冰上。一旦合并所有试剂合用于运行，应立即迅速 SignalStar 溶液。
4. Incubate slides in 150 µL of SignalStar Fluorescence Removal Solution for 2 hr at 37°C. (Use of a covered humidity chamber is highly recommended to prevent evaporation.)
5. 将载玻片浸没于 dH2O 中 30 秒。
6. Complete any additional staining and imaging steps following your manufacturer’s protocol.
For the SignalStar™ Multiplex IHC Assay for Use on BOND RX Fully Automated Research Stainer by Leica Biosystems protocol:
1. In the BOND RX software by Leica Biosystems, create a copy of the "CST SignalStar Imaging Round 2” protocol.
2. Change the name of the copy to "CST SignalStar Imaging Round 3” with the abbreviated name "CST Rd3.”
3. 选择“显示洗涤步骤”。
4. Delete Steps 8-63, saving only steps 1-7 for fluorescence removal.
5. Add steps required for any additional staining following your manufacturer’s protocol.
6. Select "CST SignalStar" as the preferred Detection System (Note: Additional staining steps may require further selection of other Detection Systems. Please contact Leica Biosystems for assistance.)
7. Select "Create Protocol.”
8. Click the Save button and then the Yes button to acknowledge the caution message.
9. After the second round of image acquisition, soak slides in dH2O for >30 min to gently remove coverslip.
10. Combine reagents to create the SignalStar Fluorescence Removal Solution:
a. For 5 slides: 185 µL 10X dsDNase Buffer, 18.5 µL dsDNase, and 1,646.5 µL Nuclease-free Water #12931 in 1 BOND Titration Insert.
b. For 10 slides: 335 µL 10X dsDNase Buffer, 33.5 µl dsDNase, and 2,981.5 µL Nuclease-free Water #12931 in 1 BOND Titration Insert.
11. 用封口膜覆盖并涡旋混合 10 秒。配制溶液时，所有 SignalStar 试剂盒组分均储存于冰上。一旦合并所有试剂合用于运行，应立即迅速 SignalStar 溶液。
12. In the BOND RX software, create a study and add slides.
13. When "adding slides," use the selections below for Tissue Preparation on BOND:
a. Slide preparation: -- (no value/not required)
b. Dispense Volume: 150 µL
c. HIER: -- (no value/not required)
14. 选择“CST SignalStar 第 3 轮成像”，确保将载玻片准备选为“--”并且将 HIER 选为“--”。
15. 打印标签，向载玻片添加标签，并将载玻片置于 BOND 载玻片托盘上。
16. 在添加 BOND 覆盖物之前，将 2-3 滴 dH2O 滴到每块载玻片上。
17. 启动 BOND RX 运行。
除非如以 CST 合法授权代表签署的书面形式另行明确同意，否则以下条款适用于 CST、其附属公司或其分销商提供的产品。除非 CST 合法授权代表以书面形式单独接受，否则任何附加于或异于此处所载条款和条件的客户条款和条件均被拒绝且无效。
产品用“仅供研究使用”或类似标示声明标示，并且尚未经 FDA 或其他国外或国内监管实体出于任何目的批准、准许或许可。客户不得出于任何诊断或治疗目的或以任何与产品标示声明相冲突的方式使用任何产品。CST 销售或许可的产品提供给作为最终用户的客户，且仅用于研究和开发用途。出于诊断、预防或治疗目的任何产品使用或出于转售（单独或作为成分）或其他商业目的的任何产品购买都要求来自 CST 的单独许可。客户 (a) 不得向任何第三方出售、许可、出借、捐赠或另行转让或提供任何本公司产品，无论单独或联合其他材料方式，或使用本公司产品制造任何商业产品，(b) 不得复制、修改、逆向工程、反编译、反汇编或另行尝试发现本公司产品的底层结构或技术，或出于开发与 CST 产品或服务竞争的任何产品或服务的目的使用本公司产品，(c) 不得从本公司产品改变或移除任何商标、商品名称、徽标、专利或版权声明或标记，(d) 仅应根据 CST 产品销售条款和任何适用文档使用本公司产品，以及 (e) 应就客户联系本公司产品所用的任何第三方产品或服务而言遵守任何许可、服务条款或类似协议。
